Day: August 26, 2019

1.Name the enzyme which is used to produce Flavr – Savr tomato.

2.Why is golden rice superior to normal rice?

3.How a future vaccine holds a promise of bypassing the need to visit the doctor regularly for childhood immunizations?

4.  Why plants produce secondary metabolites?

5. Name the gene introduced in transgenic tomato for insect resistance.

6. When substance X is added to a plant tissue culture medium, it promoted rooting whereas substance Y is added, it promoted shooting. Identify substances X and Y?

7. You have been asked by an avocado grower, to find away to genetically engineer his crop to prevent it from ripening during shipping. What experimental approach would you suggest?

8.What factors dictate the choice of plant tissue culture media?

9. Name the major medias used in PTC.

10. Who is considered as father of PTC and why?

11. What do you understand by the term second green revolution?

12. A farmer wants to produce hybrid of mustard plants in his field. As a biotechnologist, what would you suggest to him to ensure successful pollination or fertilization?

13. What is an artificial seed?

14. Weeds compete with crop plants for nutrients, water etc. and are responsible for significant reduction in crop yields. As a biotechnologist, suggest a way to overcome this problem.

15. Execute the engineered biosynthetic pathway which leads to the production of valuable secondary metabolites for their overproduction.

1.Explain how cDNA microarray technique can be used to study cellular response to the environment? Support your answer with a flowchart for the same.

2.(a) Which information can be retrieved from the following databases:

          (i) EMBL

          (ii) PDB

          (iii) PALI (b) Give two reasons for completely sequencing a genome.

3. Define SNPs. Describe a possible use of this technique in medicine. How do the physicians decide our susceptibility or resistance to a particular disease through this technique? Explain with the help of an example.

4. Gene prediction by computers is different from number of genes identified by experimental methods. Why is it so? Is there any correlation between the complexity of an organism and the total number of genes in its genome? Justify.

5. What kind of analysis can be done using Bioinformatics tools for DNA and proteins?

6. Expand BLAST. Discuss the steps involved in comparison of DNA sequences using this tool. Differentiate between paralogs and homologs.

7. Name the two methodologies used for genome sequencing. Present a comparative account.

8. CML patients have the ‘Philadelphia Chromosome”. How is this chromosome detected? Explain the technique with a diagram.

9. Breast cancer cells often exhibit abnormal expression of certain genes which are too many to study individually. Describe a method that you would use to compare the gene expression in the breast cancer cells and a normal cell.

10. Name four major databases for bioinformatics with their respective information contents. Name any database retrieval tool and its application.