BIOTECHNOLOGY: SYLLABUS OF CLASS XII

Unit-V Protein and Gene Manipulation 

Chapter-1: Recombinant DNA Technology

Introduction, Tool of rDNA technology, Making rDNA, Introduction of recombinant DNA into host cells, Identification  of  Recombinants, Polymerase Chain Reaction  (PCR), Hybridization Techniques, DNA Library, DNA Sequencing, Site-directed Mutagenesis.

Chapter-2: Protein Structure and Engineering

Introduction to the world of proteins, 3-D shape of proteins, Structure-Function Relationship in Proteins, Purification  of  Proteins,  Characterization  of  Proteins,  Protein  Based  Products, Designing Proteins (Protein Engineering).

Chapter-3: Genomics, Proteomics and Bioinformatics

Introduction, Genome, Sequencing Projects, Gene prediction and counting, Genome  Similarity, SNPs and Comparative Genomics, Functional Genomics, Proteomics, History of Bioinformatics, Sequences and nomenclature, Information Sources, Analysis using Bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation (30 Marks, 80 Periods)

Chapter-1: Microbial Cell Culture and Its Applications

Introduction, Microbial Nutrition and Culture Techniques, Measurement and Kinetics of Microbial Growth,   Scale-up   of  Microbial   Process,  Isolation   of   Microbial   Products,  Strain   Isolation  and Improvement, Applications of Microbial Culture Technology, Biosafety Issues in Microbial Technology.

Chapter-2: Plant Cell Culture and Applications

Introduction,  Cell  and  Tissue  Culture  Techniques,  Applications  of  Cell  and  Tissue  Culture,  Gene Transfer Methods in Plants, Transgenic Plants with Beneficial Traits, Biosafety of Transgenic Plants.

Chapter-3: Animal Cell Culture and Applications

Introduction, Animal Cell Culture Techniques, Characterization of Cell lines, Methods of Gene Delivery into Cells, Scale-up of Animal Culture Process, Applications of Animal Cell Culture, Stem Cell Technology, Tissue Engineering.

Practicals (30 Marks)

Note: Every student will be required to do the following experiments during the academic session.

List of Experiments

1. Use of special equipment in biotechnology experiments.

2. Isolation of bacterial plasmid DNA

3. Detection of DNA by gel electrophoreses

4. Isoloation of Genomic DNA (CTAB method)

5. Estimation of DNA

6. Bacterial transformation using any plasmid

7. Restriction digestion of plasmid DNA & its analysis by gel electrophoreses

8. Isolation of bacterial grom curd & staining of bacteria

9. Cell viability assay

10. Data retreival and data base search using internet site NCBI and download a DNA and protein sequence from internet, analyse it and comment on it

11. Reading of a DNA sequencing gel to arrive at the sequence

12. Project work

Scheme of Evaluation (Practicals):

Time 3 Hours | Maximum marks 30

A	Two experiments	6+6 (only one computer based practical)
	Practical record	04
	Viva on Practicals	04
B	Project work
	Write up	05
	Viva on project	05
	Total	30