1. Specific activity increases during subsequent steps of a protein purification scheme. How can you relate it with the purity of protein?
  2. Why is the nutrient medium autoclaved before using it for culturing microbes?
  3. What is lyophilization?
  4. Write a chemical equation for the production of products from the reactants.
  5. For strain preservation glycerol (10-30%) is added, before microbial cultures are dispensed into sealed ampoules and frozen in liquid nitrogen (-176 to -1960C). What is the role of glycerol here?
  6. How does heat labile media is sterilized?
  7. Why the pH of the culture medium is is important? In a large fermenter, how is the pH maintained?
  8. Name the technique which can measure live microbial cells.
  9. How is aeration of microbial cultures achieved in laboratory?
  10. As microbiologist, what factors dictate the choice of your media?

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