- Specific activity increases during subsequent steps of a protein purification scheme. How can you relate it with the purity of protein?
- Why is the nutrient medium autoclaved before using it for culturing microbes?
- What is lyophilization?
- Write a chemical equation for the production of products from the reactants.
- For strain preservation glycerol (10-30%) is added, before microbial cultures are dispensed into sealed ampoules and frozen in liquid nitrogen (-176 to -1960C). What is the role of glycerol here?
- How does heat labile media is sterilized?
- Why the pH of the culture medium is is important? In a large fermenter, how is the pH maintained?
- Name the technique which can measure live microbial cells.
- How is aeration of microbial cultures achieved in laboratory?
- As microbiologist, what factors dictate the choice of your media?