- In assesing the effect of growth factors on animal cell cultures, a particular phase of growth is more suitable. Name this phase giving reason for the same.
- How is lipofection used to deliver genes into cells?
- What is OKT-3? Why is it administered during kidney transplantations?
- What is embryonic stem cell culture? State their observed properties in tissue culture.
- Name the two antibiotics added to animal culture media. Why cannot these be used in microbial media?
- How are animal cells stored and how are they revived when require for culturing?
- Based on microscopic observation, how can an oncologist differentiate between cancerous and normal cells?
- Why is r- HuEPO preferred over blood transfusion in a person with blood loss due to accident?
- The long distance runners are disqualified if they test positive for EPO. What is it? What is its impact on the body of runner?
- What is the importance of maintaining pH while culturing animal cells? How is the pH maintained in a culture media?
- Karyotype determination of animal cell culture is important. Why? What factors affect its stability?
- Why is it difficult to culture animal cells as compared to plant cells? Why is it essential to supplement animal cell culture media with serum?
- Why is it possible to store animal cells for long periods at very low temperature in the presence of DMSO and high concentration of serum?
- Administration of monoclonal antibodies against CD3 can help accept renal allograft in patients. Why?
- As a student of biotechnology you know that animal cell culture media is supplemented with serum. Discuss what are advantages and limitations of using serum in the media.
- As a student of biotechnology you know that animal cell culture media is supplemented with serum. Discuss what are advantages and limitations of using serum in the media.
- Why are animal cells grown in CO2 incubators and not in regular incubators? Do we require similar incubators for culturing microbial cells?
- What is the difference between a defined and a serum-supplemented medium?
- Once a cell line has been established, its karyotype has to be determined. Why?
- With an example explain how secondary cell cultures are derived?
- Why are low speed centrifuge required for most cell culture?
- Burn victims are grafted with skin peels from other parts of their body rather than from a donor individual. Why?
- If you wish to scale up cells derived from human tissue, what kind of culture set up will you use and why?
- Expand: FSH, ICM, FGH, PDGF
- What are inverted microscopes and why are they useful in animal cell culture lab?
- Animal cells in a culture medium were placed in a regular incubator used for growing bacterial cells. Do you expect the animal cells to grow or not?
- What is meant by tissue engineering? Explain any two important medical applications of tissue engineering.
- How do we analyse the growth characteristics of a particular cell line?
Class XII Animal Cell Culture 1 mark questions
- What is meant by tissue engineering?
- Animal cells were cultured in a peptone – glucose broth. They failed to grow and died. Why?
- How can cancerous cells be detected in a biopsy?
- Why is tPA administered after cerebral hemorrhage?
- State any two features of animal cell culture.
- Why are animal cell culture media supplemented with fetal calf serum?
- What is herceptin?
- Antibiotics are added to culture medium for animal cells. Why?
- Why a pan of water is always kept in an incubator chamber, used for animal cell culture?
- Name the scientist who established the first human cell line from cervix cancer cells.
- Why is it essential to supplement animal cell culture media with serum?
- Why is humulin considered better than pig insulin for the treatment of diabetes?
- Name the scientist who were first to introduce trypsin for the sub culturing of adherent cells.
- What is Gene Knock Out?
- Why it is necessry to prepare karyotype of cell before its culture?
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